chemiscope6100 imaging system (Clinx Science)
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Chemiscope6100 Imaging System, supplied by Clinx Science, used in various techniques. Bioz Stars score: 96/100, based on 3667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chemiluminescence+imaging+system/ChemiScope+6100+chemiluminescence+and+fluorescence+imaging+system/pmc13034767-64-6-9
Average 96 stars, based on 3667 article reviews
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Imaging:Article Title: Saikosaponin D and paeoniflorin improve the HCC immune microenvironment via CLCF1/PD-L1 mediated crosstalk between CAFs and tumor cells. Article Snippet: .. Afterward, the membranes were cultivated with secondary antibodies (Table S1) at 37°C for 1.5 h. Finally, the membranes were visualized using ECL Plus super-sensitive luminescent liquid (P1050-100, Applygen, Beijing, China) and analyzed with a Article Title: DYRK1B Inhibition by AZ191 Sensitizes High-Grade Serous Ovarian Cancer to Niraparib Through Promoting Apoptosis and Ferroptosis Article Snippet: .. Protein bands were https://doi.org/10.3390/biomedicines14040939 visualized using ECL reagent, and grayscale value was detected with a Article Title: HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasion Article Snippet: .. Images were captured via a Article Title: Prolactin Drives the Pathogenic Amplification of Atopic Dermatitis by Promoting CCL17 Secretion Through Activation of the PI3K/AKT Pathway. Article Snippet: Background: Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disorder driven by a combination of epidermal barrier dysfunction, immune dysregulation, and psychological stress.. Although neuroendocrine-immune interactions are known to contribute to the pathogenesis of AD, the key endocrine mediators and their molecular mechanisms require elucidation.. Prolactin (PRL), a stress-associated hormone with cytokine-like immunomodulatory properties, has not been fully characterized in the context of AD. Article Title: Mesenchymal stem cells inhibited chronic myeloid leukemia cells in vitro, correlating with oxidative stress. Article Snippet: After sealing with skim milk, membranes were incubated overnight at 4°C with primary antibodies, including anti-Nrf2 (Proteintech, 16396-1-AP, USA), anti-IκBα (Proteintech, 10268-1-AP, USA), and anti-GAPDH (Proteintech, 10494-1-AP, USA). .. Membranes were rinsed and incubated with a AR TIC LE IN PR ES S secondary IgG antibody (Proteintech, SA00001-2, USA) for 2 h. Protein bands were detected utilizing BeyoECL Plus (Ultra-sensitive ECL Chemiluminescence Kit) (Beyotime, P0018S, China) and Article Title: Indazole derivative, and preparation method therefor and use thereof Article Snippet: After the membrane was transferred, the membrane was added in 1×QuickBlock blocking buffer (Beyotime) and incubated for 30 minutes at room temperature. .. After blocking, PVDF membranes were incubated with IRAK4 primary antibody (Abcam) overnight at 4°, the membranes were washed with TBST buffer (2.4 g Tris, 8.8 g NaCl, 1.5 mL Tween 20, pH adjusted to 7.4, constant volume to 1 L) for 30 minutes, incubated with secondary antibody (Abcam) for 2 hours at room temperature, finally incubated with Clarity Western ECL Substrate (BIO-RAD) for 5 minutes for luminescence development, Article Title: Dual-targeting of mitochondrial and c-Myc G-quadruplexes by a photoactive coumarin-benzothiazolium conjugate inhibits triple-negative breast cancer cell growth. Article Snippet: Targeting G-quadruplexes (G4s) presents a promising strategy for cancer therapy, but early-generation ligands often lack tumor selectivity.. This study identified X-3, a novel photoactive coumarin-benzothiazolium conjugate, as a dual-targeted photosensitizer against triple-negative breast cancer (TNBC).. X-3 localized to both mitochondria and the nucleus, where it specifically bound to mitochondrial and c-Myc G4s with high affinity. Article Title: iPSC-derived exosomes promote diabetic wound healing by attenuating inflammatory responses. Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS (M21002S, Abmart, China) for 1 hour at room temperature. .. The blots were visualized using a Incubation:Article Title: Mesenchymal stem cells inhibited chronic myeloid leukemia cells in vitro, correlating with oxidative stress. Article Snippet: After sealing with skim milk, membranes were incubated overnight at 4°C with primary antibodies, including anti-Nrf2 (Proteintech, 16396-1-AP, USA), anti-IκBα (Proteintech, 10268-1-AP, USA), and anti-GAPDH (Proteintech, 10494-1-AP, USA). .. Membranes were rinsed and incubated with a AR TIC LE IN PR ES S secondary IgG antibody (Proteintech, SA00001-2, USA) for 2 h. Protein bands were detected utilizing BeyoECL Plus (Ultra-sensitive ECL Chemiluminescence Kit) (Beyotime, P0018S, China) and Article Title: Indazole derivative, and preparation method therefor and use thereof Article Snippet: After the membrane was transferred, the membrane was added in 1×QuickBlock blocking buffer (Beyotime) and incubated for 30 minutes at room temperature. .. After blocking, PVDF membranes were incubated with IRAK4 primary antibody (Abcam) overnight at 4°, the membranes were washed with TBST buffer (2.4 g Tris, 8.8 g NaCl, 1.5 mL Tween 20, pH adjusted to 7.4, constant volume to 1 L) for 30 minutes, incubated with secondary antibody (Abcam) for 2 hours at room temperature, finally incubated with Clarity Western ECL Substrate (BIO-RAD) for 5 minutes for luminescence development, Blocking Assay:Article Title: Indazole derivative, and preparation method therefor and use thereof Article Snippet: After the membrane was transferred, the membrane was added in 1×QuickBlock blocking buffer (Beyotime) and incubated for 30 minutes at room temperature. .. After blocking, PVDF membranes were incubated with IRAK4 primary antibody (Abcam) overnight at 4°, the membranes were washed with TBST buffer (2.4 g Tris, 8.8 g NaCl, 1.5 mL Tween 20, pH adjusted to 7.4, constant volume to 1 L) for 30 minutes, incubated with secondary antibody (Abcam) for 2 hours at room temperature, finally incubated with Clarity Western ECL Substrate (BIO-RAD) for 5 minutes for luminescence development, Western Blot:Article Title: Indazole derivative, and preparation method therefor and use thereof Article Snippet: After the membrane was transferred, the membrane was added in 1×QuickBlock blocking buffer (Beyotime) and incubated for 30 minutes at room temperature. .. After blocking, PVDF membranes were incubated with IRAK4 primary antibody (Abcam) overnight at 4°, the membranes were washed with TBST buffer (2.4 g Tris, 8.8 g NaCl, 1.5 mL Tween 20, pH adjusted to 7.4, constant volume to 1 L) for 30 minutes, incubated with secondary antibody (Abcam) for 2 hours at room temperature, finally incubated with Clarity Western ECL Substrate (BIO-RAD) for 5 minutes for luminescence development, |
